The paternal relationship between Isaac Muxlow and his son Elmer Harold Muxlow is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JS.

The segmental (via DNA segment triangulation) and total DNA shared between JS and other confirmed descendants of Isaac Muxlow/Sarah Rippin is consistent with the expected ranges for their respective relationships. Since JS descends from Isaac Muxlow/Sarah Rippin through Isaac Muxlow and his son Elmer Harold Muxlow, the presence of significant shared DNA between JS and other known descendants of Isaac Muxlow/Sarah Rippin validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Isaac Muxlow/Sarah Rippin through Isaac Muxlow to Elmer Harold Muxlow, and ultimately to JS, confirming this direct ancestral connection through multiple generations.