The proposed relationship of James Crowley as the father of Martin Crowley and Anne Crowley is supported by DNA evidence indicating shared ancestry within the family lines.

Specifically, DNA evidence confirms a 3rd cousin, once removed relationship between AFF (a descendant of the Anne Crowley ancestral line) and EM (a descendant of the Martin Crowley ancestral line) with a shared segment of 100.09 cM across 4 HIR segments.

This match is consistent with their placement within these shared ancestral branches.