The maternal relationship between Anne Crowley and her son Patrick Comyns is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant AFF.

The segmental (via DNA segment triangulation) and total DNA shared between AFF and other confirmed descendants of JC is consistent with the expected ranges for their respective relationships. Since AFF descends from JC through Anne Crowley and her son Patrick Comyns, the presence of significant shared DNA between AFF and other known descendants of JC validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA JC through Anne Crowley to Patrick Comyns, and ultimately to AFF, confirming this direct ancestral connection through multiple generations.