The proposed relationship of John Gilbert Chambers as the father of Lillian Christie Chambers Curran and Mildred Catherine Chambers is supported by DNA evidence indicating shared ancestry within the family lines.

Specifically, DNA evidence confirms a 1st cousin, twice removed relationship between NPNBB (a descendant of the Mildred Catherine Chambers ancestral line) and PCD (a descendant of the Lillian Christie Chambers Curran ancestral line) with a shared segment of 227.38 cM across 10 HIR segments.

This match is consistent with their placement within these shared ancestral branches.