The maternal relationship between Mildred Catherine Chambers and her daughter MMHBHB is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant NPNBB.

The segmental (via DNA segment triangulation) and total DNA shared between NPNBB and other confirmed descendants of John Gilbert Chambers/Mariannne Marion Mary Anne Veronica McDermott is consistent with the expected ranges for their respective relationships. Since NPNBB descends from John Gilbert Chambers/Mariannne Marion Mary Anne Veronica McDermott through Mildred Catherine Chambers and her daughter MMHBHB, the presence of significant shared DNA between NPNBB and other known descendants of John Gilbert Chambers/Mariannne Marion Mary Anne Veronica McDermott validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple John Gilbert Chambers/Mariannne Marion Mary Anne Veronica McDermott through Mildred Catherine Chambers to MMHBHB, and ultimately to NPNBB, confirming this direct ancestral connection through multiple generations.