The paternal relationship between Hanson Bowles and his daughter Mary Luna Bowles is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JKH.

The segmental (via DNA segment triangulation) and total DNA shared between JKH and other confirmed descendants of TB/BS is consistent with the expected ranges for their respective relationships. Since JKH descends from TB/BS through Hanson Bowles and his daughter Mary Luna Bowles, the presence of significant shared DNA between JKH and other known descendants of TB/BS validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple TB/BS through Hanson Bowles to Mary Luna Bowles, and ultimately to JKH, confirming this direct ancestral connection through multiple generations.