The maternal relationship between Martha Rahn and her son James Pakenham is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant CS.

The segmental (via DNA segment triangulation) and total DNA shared between CS and other confirmed descendants of CFAR/USMT is consistent with the expected ranges for their respective relationships. Since CS descends from CFAR/USMT through Martha Rahn and her son James Pakenham, the presence of significant shared DNA between CS and other known descendants of CFAR/USMT validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple CFAR/USMT through Martha Rahn to James Pakenham, and ultimately to CS, confirming this direct ancestral connection through multiple generations.