The maternal relationship between Eliza Muxlow and her son WA is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JA.

The segmental (via DNA segment triangulation) and total DNA shared between JA and other confirmed descendants of Isaac Muxlow/Sarah Rippin is consistent with the expected ranges for their respective relationships. Since JA descends from Isaac Muxlow/Sarah Rippin through Eliza Muxlow and her son WA, the presence of significant shared DNA between JA and other known descendants of Isaac Muxlow/Sarah Rippin validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Isaac Muxlow/Sarah Rippin through Eliza Muxlow to WA, and ultimately to JA, confirming this direct ancestral connection through multiple generations.