The proposed relationship of Isaac M. Glasford as the father of Ida Belle "Minnie" Glasford and Marietta "Mary" Glasford is supported by DNA evidence indicating shared ancestry within the family lines.

Specifically, DNA evidence confirms a distant cousin relationship between RDM (a descendant of the Ida Belle "Minnie" Glasford ancestral line) and CHS (a descendant of the Marietta "Mary" Glasford ancestral line) with a shared segment of 46.08 cM across 4 HIR segments.

This match is consistent with their placement within these shared ancestral branches.