The maternal relationship between Josephine Margaret Lennox and her daughter DDF is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant KLF.

The segmental (via DNA segment triangulation) and total DNA shared between KLF and other confirmed descendants of John Raleigh Lennox/Florence O'Brien is consistent with the expected ranges for their respective relationships. Since KLF descends from John Raleigh Lennox/Florence O'Brien through Josephine Margaret Lennox and her daughter DDF, the presence of significant shared DNA between KLF and other known descendants of John Raleigh Lennox/Florence O'Brien validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple John Raleigh Lennox/Florence O'Brien through Josephine Margaret Lennox to DDF, and ultimately to KLF, confirming this direct ancestral connection through multiple generations.