The maternal relationship between Opal Lorraine Dubree and her son MB is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant MB.

The segmental (via DNA segment triangulation) and total DNA shared between MB and other confirmed descendants of James Chapman/Ruth Bertha Hammer is consistent with the expected ranges for their respective relationships. Since MB descends from James Chapman/Ruth Bertha Hammer through Opal Lorraine Dubree and her son MB, the presence of significant shared DNA between MB and other known descendants of James Chapman/Ruth Bertha Hammer validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple James Chapman/Ruth Bertha Hammer through Opal Lorraine Dubree to MB, and ultimately to MB, confirming this direct ancestral connection through multiple generations.