The paternal relationship between John Thomas Axtell and his daughter Elizabeth Axtell is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant PT.

The segmental (via DNA segment triangulation) and total DNA shared between PT and other confirmed descendants of Thomas Axtell/Mary Tuttle is consistent with the expected ranges for their respective relationships. Since PT descends from Thomas Axtell/Mary Tuttle through John Thomas Axtell and his daughter Elizabeth Axtell, the presence of significant shared DNA between PT and other known descendants of Thomas Axtell/Mary Tuttle validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Thomas Axtell/Mary Tuttle through John Thomas Axtell to Elizabeth Axtell, and ultimately to PT, confirming this direct ancestral connection through multiple generations.