The paternal relationship between Robert Francis Falardeau and his daughter LO is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant LO.

The segmental (via DNA segment triangulation) and total DNA shared between LO and other confirmed descendants of John Aloysius O'Brien/Felicite Prairie is consistent with the expected ranges for their respective relationships. Since LO descends from John Aloysius O'Brien/Felicite Prairie through Robert Francis Falardeau and his daughter LO, the presence of significant shared DNA between LO and other known descendants of John Aloysius O'Brien/Felicite Prairie validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple John Aloysius O'Brien/Felicite Prairie through Robert Francis Falardeau to LO, and ultimately to LO, confirming this direct ancestral connection through multiple generations.