The maternal relationship between Mary Elizabeth Johnson and her son Alvin Vernett Boylan is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant SRB.

The segmental (via DNA segment triangulation) and total DNA shared between SRB and other confirmed descendants of Isaac Johnson/Rachel Catherine Robinson is consistent with the expected ranges for their respective relationships. Since SRB descends from Isaac Johnson/Rachel Catherine Robinson through Mary Elizabeth Johnson and her son Alvin Vernett Boylan, the presence of significant shared DNA between SRB and other known descendants of Isaac Johnson/Rachel Catherine Robinson validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Isaac Johnson/Rachel Catherine Robinson through Mary Elizabeth Johnson to Alvin Vernett Boylan, and ultimately to SRB, confirming this direct ancestral connection through multiple generations.