The maternal relationship between Nellie Rebecca Lukens and her daughter HLG is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant KR.

The segmental (via DNA segment triangulation) and total DNA shared between KR and other confirmed descendants of Milton D. Lukens/Lydia Ann Suplee is consistent with the expected ranges for their respective relationships. Since KR descends from Milton D. Lukens/Lydia Ann Suplee through Nellie Rebecca Lukens and her daughter HLG, the presence of significant shared DNA between KR and other known descendants of Milton D. Lukens/Lydia Ann Suplee validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Milton D. Lukens/Lydia Ann Suplee through Nellie Rebecca Lukens to HLG, and ultimately to KR, confirming this direct ancestral connection through multiple generations.