The maternal relationship between Dorothea Sophia Ramthun and her daughter Anna Gaulke is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JP.

The segmental (via DNA segment triangulation) and total DNA shared between JP and other confirmed descendants of Martin Ramthun is consistent with the expected ranges for their respective relationships. Since JP descends from Martin Ramthun through Dorothea Sophia Ramthun and her daughter Anna Gaulke, the presence of significant shared DNA between JP and other known descendants of Martin Ramthun validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA Martin Ramthun through Dorothea Sophia Ramthun to Anna Gaulke, and ultimately to JP, confirming this direct ancestral connection through multiple generations.