The paternal relationship between Ludwig Hübner and his daughter Alvine Hübner is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant RR.

The segmental (via DNA segment triangulation) and total DNA shared between RR and other confirmed descendants of Gottlieb Benjamin HÜBNER/Johanna Susanna Schulz is consistent with the expected ranges for their respective relationships. Since RR descends from Gottlieb Benjamin HÜBNER/Johanna Susanna Schulz through Ludwig Hübner and his daughter Alvine Hübner, the presence of significant shared DNA between RR and other known descendants of Gottlieb Benjamin HÜBNER/Johanna Susanna Schulz validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Gottlieb Benjamin HÜBNER/Johanna Susanna Schulz through Ludwig Hübner to Alvine Hübner, and ultimately to RR, confirming this direct ancestral connection through multiple generations.