The paternal relationship between James Dillon Humphrey and his son Hugh Humphrey is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JH.

The segmental (via DNA segment triangulation) and total DNA shared between JH and other confirmed descendants of GH/Mary Luna Bowles is consistent with the expected ranges for their respective relationships. Since JH descends from GH/Mary Luna Bowles through James Dillon Humphrey and his son Hugh Humphrey, the presence of significant shared DNA between JH and other known descendants of GH/Mary Luna Bowles validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple GH/Mary Luna Bowles through James Dillon Humphrey to Hugh Humphrey, and ultimately to JH, confirming this direct ancestral connection through multiple generations.