The paternal relationship between John Tompkins and his son Nathaniel Tompkins is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JZ.

The segmental (via DNA segment triangulation) and total DNA shared between JZ and other confirmed descendants of John Tompkins II/Rebecca Knight is consistent with the expected ranges for their respective relationships. Since JZ descends from John Tompkins II/Rebecca Knight through John Tompkins and his son Nathaniel Tompkins, the presence of significant shared DNA between JZ and other known descendants of John Tompkins II/Rebecca Knight validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple John Tompkins II/Rebecca Knight through John Tompkins to Nathaniel Tompkins, and ultimately to JZ, confirming this direct ancestral connection through multiple generations.