The proposed relationship of Johann Baptist Fessler as the father of Charles Theodore Fessler, Maria Fessler and Margreth Fessler is supported by DNA evidence indicating shared ancestry within the family lines.

Specifically, DNA evidence confirms a 3rd cousin, once removed relationship between KLF (a descendant of the Charles Theodore Fessler ancestral line) and LB (a descendant of the Maria Fessler ancestral line) with a shared segment of 52.35 cM across 3 HIR segments.

It also confirms a 3rd cousin, once removed relationship between KLF (a descendant of the Charles Theodore Fessler ancestral line) and GJ (a descendant of the Margreth Fessler ancestral line) with a shared segment of 87.13 cM across 5 HIR segments.

These matches are consistent with their placement within these shared ancestral branches.