The maternal relationship between Johanna Auguste Mathilde Radtke and her daughter Marie Helen Arndt is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant CS.

The segmental (via DNA segment triangulation) and total DNA shared between CS and other confirmed descendants of Christian Ludwig Glander/Anna Christina Wojahn is consistent with the expected ranges for their respective relationships. Since CS descends from Christian Ludwig Glander/Anna Christina Wojahn through Johanna Auguste Mathilde Radtke and her daughter Marie Helen Arndt, the presence of significant shared DNA between CS and other known descendants of Christian Ludwig Glander/Anna Christina Wojahn validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Christian Ludwig Glander/Anna Christina Wojahn through Johanna Auguste Mathilde Radtke to Marie Helen Arndt, and ultimately to CS, confirming this direct ancestral connection through multiple generations.