The maternal relationship between Marion Elizabeth Cain and her daughter PKH is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant PKH.

The segmental (via DNA segment triangulation) and total DNA shared between PKH and other confirmed descendants of Bernard "barney" R Cain is consistent with the expected ranges for their respective relationships. Since PKH descends from Bernard "barney" R Cain through Marion Elizabeth Cain and her daughter PKH, the presence of significant shared DNA between PKH and other known descendants of Bernard "barney" R Cain validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA Bernard "barney" R Cain through Marion Elizabeth Cain to PKH, and ultimately to PKH, confirming this direct ancestral connection through multiple generations.