The maternal relationship between Margaret Frances King and her son JO is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JO.

The segmental (via DNA segment triangulation) and total DNA shared between JO and other confirmed descendants of BG/S is consistent with the expected ranges for their respective relationships. Since JO descends from BG/S through Margaret Frances King and her son JO, the presence of significant shared DNA between JO and other known descendants of BG/S validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple BG/S through Margaret Frances King to JO, and ultimately to JO, confirming this direct ancestral connection through multiple generations.