The paternal relationship between Jacob R Brashears and his son John Pendleton Brashears is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JPS.

The segmental (via DNA segment triangulation) and total DNA shared between JPS and other confirmed descendants of Robert Brashears/Sarah Rhea Hankins is consistent with the expected ranges for their respective relationships. Since JPS descends from Robert Brashears/Sarah Rhea Hankins through Jacob R Brashears and his son John Pendleton Brashears, the presence of significant shared DNA between JPS and other known descendants of Robert Brashears/Sarah Rhea Hankins validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Robert Brashears/Sarah Rhea Hankins through Jacob R Brashears to John Pendleton Brashears, and ultimately to JPS, confirming this direct ancestral connection through multiple generations.