The proposed relationship of Squire Turner Estes as the father of Steven Matthew Estes and Pleasant Henderson Estes is supported by DNA evidence indicating shared ancestry within the family lines.

Specifically, DNA evidence confirms a 2nd cousin, once removed relationship between MEP (a descendant of the Pleasant Henderson Estes ancestral line) and KS (a descendant of the Steven Matthew Estes ancestral line) with a shared segment of 103.54 cM .

This match is consistent with their placement within these shared ancestral branches.