The maternal relationship between Wilhemine Augusta Grade and her daughter Hilda Chrispens is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant AF.

The segmental (via DNA segment triangulation) and total DNA shared between AF and other confirmed descendants of AG/Louise Wilhelmine Glander is consistent with the expected ranges for their respective relationships. Since AF descends from AG/Louise Wilhelmine Glander through Wilhemine Augusta Grade and her daughter Hilda Chrispens, the presence of significant shared DNA between AF and other known descendants of AG/Louise Wilhelmine Glander validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple AG/Louise Wilhelmine Glander through Wilhemine Augusta Grade to Hilda Chrispens, and ultimately to AF, confirming this direct ancestral connection through multiple generations.